MYO10-filopodia support basement membranes at pre-invasive tumor boundaries

Breast cancer organoid (magenta) invading through collagen (green). Credit : Emilia Peuhu and Guillaume Jacquemet.

Ductal carcinoma in situ (DCIS) is a pre-invasive stage of breast cancer. During invasion, the encapsulating DCIS basement membrane (BM) is compromised, and tumor cells invade the surrounding stroma. The mechanisms that regulate functional epithelial BMs in vivo are poorly understood. Myosin-X (MYO10) is a filopodia-inducing protein associated with metastasis and poor clinical outcome in invasive breast cancer (IBC). We identify elevated MYO10 expression in human DCIS and IBC, and this suggests links with disease progression. MYO10 promotes filopodia formation and cell invasion in vitro and cancer-cell dissemination from progressively invasive human DCIS xenografts. However, MYO10-depleted xenografts are more invasive. These lesions exhibit compromised BMs, poorly defined borders, and increased cancer-cell dispersal and EMT-marker-positive cells. In addition, cancer spheroids are dependent on MYO10-filopodia to generate a near-continuous extracellular matrix boundary. Thus, MYO10 is protective in early-stage breast cancer, correlating with tumor-limiting BMs, and pro-invasive at later stages, facilitating cancer-cell dissemination.

Emilia Peuhu, Guillaume Jacquemet, Colinda L.G.J. Scheele, Aleksi Isomursu, Marie-Catherine Laisne, Leena M. Koskinen, Ilkka Paatero, Kerstin Thol, Maria Georgiadou, Camilo Guzmán, Satu Koskinen, Asta Laiho, Laura L. Elo, Pia Boström, Pauliina Hartiala, Jacco van Rheenen, Johanna Ivaska

Read our paper here

Create a website or blog at